Grasshopper

algorithmic modeling for Rhino

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Primer Design

Component Index > Alba > Utilities > Primer Design

to find a primer with good melt temp and GC content and avoiding bad hairpins

Inputs

NameIDDescriptionType
TextDNAContains a collection of text fragmentsString
CurveCrvContains a collection of generic curvesCurve
IntegerStContains a collection of integer numbersInteger
IntegerLContains a collection of integer numbersInteger
IntegerShContains a collection of integer numbersInteger

Outputs

NameIDDescriptionType
PrimerPResulting textString
TempTtarget melt temp is 55-65, this reading is onoy accurate around 20-40 nucleotidesGoo
GC%GC%The result of the MultiplicationGoo
Hair Pin CountsHPhair pin counts at different lengthsGoo

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